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- Antibody Data
- Antigen structure
- References [26]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- AF2225 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse NKp46/NCR1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) NKp46 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8C567- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice.
Forced Expression of CXCL10 Prevents Liver Metastasis of Colon Carcinoma Cells by the Recruitment of Natural Killer Cells.
Therapeutic Potential of Leelamine, a Novel Inhibitor of Androgen Receptor and Castration-Resistant Prostate Cancer.
Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment.
NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression.
Preclinical, non-genetic models of lung adenocarcinoma: a comparative survey.
Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization.
NK cell heparanase controls tumor invasion and immune surveillance.
Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells.
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.
Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival.
Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells.
IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo.
IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-γ.
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure.
Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke.
BCMab1, a monoclonal antibody against aberrantly glycosylated integrin α3β1, has potent antitumor activity of bladder cancer in vivo.
TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice.
Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells.
CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells.
Nishina S, Yamauchi A, Kawaguchi T, Kaku K, Goto M, Sasaki K, Hara Y, Tomiyama Y, Kuribayashi F, Torimura T, Hino K
Cellular and molecular gastroenterology and hepatology 2019;7(1):115-134
Cellular and molecular gastroenterology and hepatology 2019;7(1):115-134
Forced Expression of CXCL10 Prevents Liver Metastasis of Colon Carcinoma Cells by the Recruitment of Natural Killer Cells.
Kikuchi N, Ye J, Hirakawa J, Kawashima H
Biological & pharmaceutical bulletin 2019 Jan 1;42(1):57-65
Biological & pharmaceutical bulletin 2019 Jan 1;42(1):57-65
Therapeutic Potential of Leelamine, a Novel Inhibitor of Androgen Receptor and Castration-Resistant Prostate Cancer.
Singh KB, Ji X, Singh SV
Molecular cancer therapeutics 2018 Oct;17(10):2079-2090
Molecular cancer therapeutics 2018 Oct;17(10):2079-2090
Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment.
Martin A, Seignez C, Racoeur C, Isambert N, Mabrouk N, Scagliarini A, Reveneau S, Arnould L, Bettaieb A, Jeannin JF, Paul C
Oncotarget 2018 Jun 19;9(47):28364-28378
Oncotarget 2018 Jun 19;9(47):28364-28378
NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression.
Turchinovich G, Ganter S, Bärenwaldt A, Finke D
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 1;200(11):3762-3768
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 1;200(11):3762-3768
Preclinical, non-genetic models of lung adenocarcinoma: a comparative survey.
Janker F, Weder W, Jang JH, Jungraithmayr W
Oncotarget 2018 Jul 17;9(55):30527-30538
Oncotarget 2018 Jul 17;9(55):30527-30538
Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization.
Er EE, Valiente M, Ganesh K, Zou Y, Agrawal S, Hu J, Griscom B, Rosenblum M, Boire A, Brogi E, Giancotti FG, Schachner M, Malladi S, Massagué J
Nature cell biology 2018 Aug;20(8):966-978
Nature cell biology 2018 Aug;20(8):966-978
NK cell heparanase controls tumor invasion and immune surveillance.
Putz EM, Mayfosh AJ, Kos K, Barkauskas DS, Nakamura K, Town L, Goodall KJ, Yee DY, Poon IK, Baschuk N, Souza-Fonseca-Guimaraes F, Hulett MD, Smyth MJ
The Journal of clinical investigation 2017 Jun 30;127(7):2777-2788
The Journal of clinical investigation 2017 Jun 30;127(7):2777-2788
Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells.
Krueger PD, Narayanan S, Surette FA, Brown MG, Sung SJ, Hahn YS
Journal of leukocyte biology 2017 Jan;101(1):329-338
Journal of leukocyte biology 2017 Jan;101(1):329-338
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.
Sung SJ, Ge Y, Dai C, Wang H, Fu SM, Sharma R, Hahn YS, Yu J, Le TH, Okusa MD, Bolton WK, Lawler JR
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601
Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival.
Rabacal W, Pabbisetty SK, Hoek KL, Cendron D, Guo Y, Maseda D, Sebzda E
Proceedings of the National Academy of Sciences of the United States of America 2016 May 10;113(19):5370-5
Proceedings of the National Academy of Sciences of the United States of America 2016 May 10;113(19):5370-5
Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells.
Luu TT, Ganesan S, Wagner AK, Sarhan D, Meinke S, Garbi N, Hämmerling G, Alici E, Kärre K, Chambers BJ, Höglund P, Kadri N
Scientific reports 2016 Dec 1;6:37996
Scientific reports 2016 Dec 1;6:37996
IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo.
Kritikou JS, Dahlberg CI, Baptista MA, Wagner AK, Banerjee PP, Gwalani LA, Poli C, Panda SK, Kärre K, Kaech SM, Wermeling F, Andersson J, Orange JS, Brauner H, Westerberg LS
Scientific reports 2016 Aug 1;6:30636
Scientific reports 2016 Aug 1;6:30636
IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-γ.
Guo Y, Luan L, Rabacal W, Bohannon JK, Fensterheim BA, Hernandez A, Sherwood ER
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2353-64
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2353-64
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
Spada R, Rojas JM, Pérez-Yagüe S, Mulens V, Cannata-Ortiz P, Bragado R, Barber DF
Journal of leukocyte biology 2015 Mar;97(3):583-98
Journal of leukocyte biology 2015 Mar;97(3):583-98
Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure.
Stolberg VR, Martin B, Mancuso P, Olszewski MA, Freeman CM, Curtis JL, Chensue SW
The American journal of pathology 2014 Feb;184(2):454-63
The American journal of pathology 2014 Feb;184(2):454-63
Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke.
Mracsko E, Liesz A, Stojanovic A, Lou WP, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Dec 10;34(50):16784-95
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Dec 10;34(50):16784-95
BCMab1, a monoclonal antibody against aberrantly glycosylated integrin α3β1, has potent antitumor activity of bladder cancer in vivo.
Li C, Yang Z, Du Y, Tang H, Chen J, Hu D, Fan Z
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Aug 1;20(15):4001-13
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Aug 1;20(15):4001-13
TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke CT, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lépinasse F, Marvel J, Verhoeyen E, Cosset FL, Peeper D, Smyth MJ, Londoño-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec JY, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
Nature cell biology 2013 Jul;15(7):818-28
Nature cell biology 2013 Jul;15(7):818-28
Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
Klezovich-Bénard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
PLoS pathogens 2012 Jan;8(1):e1002481
PLoS pathogens 2012 Jan;8(1):e1002481
Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3096-103
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3096-103
Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B
Proceedings of the National Academy of Sciences of the United States of America 2010 May 25;107(21):9759-64
Proceedings of the National Academy of Sciences of the United States of America 2010 May 25;107(21):9759-64
Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice.
Brauner H, Elemans M, Lemos S, Broberger C, Holmberg D, Flodström-Tullberg M, Kärre K, Höglund P
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2272-80
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2272-80
Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6489-99
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6489-99
Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells.
Kang SJ, Liang HE, Reizis B, Locksley RM
Immunity 2008 Nov 14;29(5):819-33
Immunity 2008 Nov 14;29(5):819-33
CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells.
Vosshenrich CA, Lesjean-Pottier S, Hasan M, Richard-Le Goff O, Corcuff E, Mandelboim O, Di Santo JP
The Journal of experimental medicine 2007 Oct 29;204(11):2569-78
The Journal of experimental medicine 2007 Oct 29;204(11):2569-78
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NKp46/NCR1 in Mouse Spleen. NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of NKp46/NCR1 in Mouse DX5/CD49b+ Splenocytes by Flow Cytometry. Mouse DX5/CD49b+ splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells. Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-gamma ELISA Kit (Catalog # MIF00). The ED50 for this effect is typically 0.4-2.4 μg/mL.
