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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NBP2-02667 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HID1 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references HID-1 controls formation of large dense core vesicles by influencing cargo sorting and trans-Golgi network acidification.
Hummer BH, de Leeuw NF, Burns C, Chen L, Joens MS, Hosford B, Fitzpatrick JAJ, Asensio CS
Molecular biology of the cell 2017 Dec 15;28(26):3870-3880
Molecular biology of the cell 2017 Dec 15;28(26):3870-3880
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HID1 Antibody (2F4) [NBP2-02667] - Analysis of extracts (35ug) from 9 different cell lines by using anti-C17orf28 monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HID1 Antibody (OTI2F4) [NBP2-02667] - Analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human prostate tissue using anti-C17orf28 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human liver tissue using anti-C17orf28 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C17orf28 antibody, and then analyzed by flow cytometry.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - Analysis of Jurkat cells, using anti-C17orf28 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
