PA5-28578

antibody from Invitrogen Antibodies

Targeting: AK4 AK3, AK3L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28578

Provider

Invitrogen Antibodies

Product name

Adenylate Kinase 4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HepG2, HCT116.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-28578). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2. Lane C: HCT116. 12% SDS PAGE. Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-28578) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-28578). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2. Lane C: HCT116. 12% SDS PAGE. Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-28578) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Adenylate Kinase 4 Polyclonal Antibody detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Adenylate kinase 4 protein stained by Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-28578) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Adenylate Kinase 4 Polyclonal Antibody detects Adenylate kinase 4 protein at mitochondria in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-28578) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Adenylate kinase 4 (Product # PA5-28578) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Adenylate kinase 4 (Product # PA5-28578) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.