Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-76341 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACOX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACOX1 in extract of various cells. Samples were incubated with ACOX1 polyclonal antibody (Product # PA5-76341).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ACOX1 Polyclonal Antibody (Product # PA5-76341) and 72 kDa, 51 kDa bands corresponding to ACOX1 was observed in cell lines and tissues tested except in Mouse Cerebellum, which is reported negative for ACOX1 expression for both isoforms and the 51 kDa isoform in Mouse Lung, a low expressing model for the same. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), tissue extracts of Mouse Lung (Lane 2), Mouse Cerebellum (Lane 3), Mouse Kidney (Lane 4), Mouse Liver (Lane 5) and Rat Liver (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). The blot was probed with the primary antibody (1:1200 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ACOX1 in paraffin-embedded mouse brain. Samples were incubated with ACOX1 polyclonal antibody (Product # PA5-76341) at a dilution of 1:100. Results were magnified with a 40x lens.