Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-21311 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GALR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GALR2 Polyclonal Antibody (Product # PA5-21311). Sample (30 µg of whole cell lysate). A: A431. 12% SDS PAGE. GALR2 Polyclonal Antibody (Product # PA5-21311) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GALR2 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GALR2 Polyclonal Antibody (Product # PA5-21311) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Galanin Receptor 2 antibody detects Galanin Receptor 2 protein at membrane and cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Galanin Receptor 2 antibody (Product # PA5-21311) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Galanin Receptor 2 antibody detects GALR2 protein at membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Galanin Receptor 2 antibody (Product # PA5-21311) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.