Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- orb137875 - Provider product page
- Provider
- Biorbyt
- Product name
- SPHK1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal to SPHK1. SPHK1 (Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al. (2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al. (1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al. (2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 μg
- Storage
- Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: RAJI Cell Lysate, Lane 2: MCF-7 Cell Lysate, Lane 3: HUT Cell Lysate, Lane 4: HELA Cell Lysate, Lane 5: 293T Cell Lysate using SPHK1 antibody
Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- Confocal immunofluoroscence analysis of HELA cells using HSD17B1 antibodySPHK1 antibody
Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed paraffin embedded Human Placenta Tissue using SPHK1 antibody