Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- F50218 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SPHK1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 59-89 from the human protein was used as the immunogen for this SPHK1 antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the SPHK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 1) HepG2 cell line and human 2) heart, 3) skeletal muscle, 4) kidney tissue using SPHK1 antibody at 1:1000. Predicted molecular weight: 42-51 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human kidney section using SPHK1 antibody; Ab was diluted at 1:100.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse kidney section using SPHK1 antibody; Ab was diluted at 1:100 dilution.