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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00008877-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008877-M01, RRID:AB_607088
- Product name
- SPHK1 monoclonal antibody (M01), clone 1D6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SPHK1.
- Antigen sequence
MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFR
SHVQPLLAEAEISFTLMLTERRNHARELVRSEELG
RWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPL
CSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTL
LLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIA
DVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRL
AYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPV
PSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRC
AAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYE
CPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAV
QGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL- Isotype
- IgG
- Antibody clone number
- 1D6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references LRIG1 modulates aggressiveness of head and neck cancers by regulating EGFR-MAPK-SPHK1 signaling and extracellular matrix remodeling.
MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1.
Factor-Xa-induced mitogenesis and migration require sphingosine kinase activity and S1P formation in human vascular smooth muscle cells.
Effect of HFE variants on sphingolipid expression by SH-SY5Y human neuroblastoma cells.
The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen.
The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line.
Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9.
A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.
Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells.
Sheu JJ, Lee CC, Hua CH, Li CI, Lai MT, Lee SC, Cheng J, Chen CM, Chan C, Chao SC, Chen JY, Chang JY, Lee CH
Oncogene 2014 Mar 13;33(11):1375-84
Oncogene 2014 Mar 13;33(11):1375-84
MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1.
Zhang H, Wang Q, Zhao Q, Di W
Journal of ovarian research 2013 Nov 26;6(1):84
Journal of ovarian research 2013 Nov 26;6(1):84
Factor-Xa-induced mitogenesis and migration require sphingosine kinase activity and S1P formation in human vascular smooth muscle cells.
Böhm A, Flößer A, Ermler S, Fender AC, Lüth A, Kleuser B, Schrör K, Rauch BH
Cardiovascular research 2013 Aug 1;99(3):505-13
Cardiovascular research 2013 Aug 1;99(3):505-13
Effect of HFE variants on sphingolipid expression by SH-SY5Y human neuroblastoma cells.
Ali-Rahmani F, Hengst JA, Connor JR, Schengrund CL
Neurochemical research 2011 Sep;36(9):1687-96
Neurochemical research 2011 Sep;36(9):1687-96
The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen.
Schröder M, Richter C, Juan MH, Maltusch K, Giegold O, Quintini G, Pfeilschifter JM, Huwiler A, Radeke HH
Molecular immunology 2011 May;48(9-10):1139-48
Molecular immunology 2011 May;48(9-10):1139-48
The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line.
Asher V, Warren A, Shaw R, Sowter H, Bali A, Khan R
Cancer cell international 2011 Mar 10;11:6
Cancer cell international 2011 Mar 10;11:6
Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9.
Schenten V, Melchior C, Steinckwich N, Tschirhart EJ, Bréchard S
Journal of leukocyte biology 2011 Apr;89(4):587-96
Journal of leukocyte biology 2011 Apr;89(4):587-96
A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.
Wang S, Zhang Z, Lin X, Xu DS, Feng Y, Ding K
Glycobiology 2010 Jan;20(4):473-84
Glycobiology 2010 Jan;20(4):473-84
Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells.
Billich A, Urtz N, Reuschel R, Baumruker T
The international journal of biochemistry & cell biology 2009 Jul;41(7):1547-55
The international journal of biochemistry & cell biology 2009 Jul;41(7):1547-55
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SPHK1 expression in transfected 293T cell line by SPHK1 monoclonal antibody (M01), clone 1D6.Lane 1: SPHK1 transfected lysate(43.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SPHK1 monoclonal antibody (M01), clone 1D6. Western Blot analysis of SPHK1 expression in K-562.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SPHK1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
