GTX129488

antibody from GeneTex

Targeting: ATG4C APG4C, AUTL1, AUTL3, FLJ14867

Western blot (Enhanced validation)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX129488

Provider

GeneTex

Product name

ATG4C antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG4C antibody (GTX129488) diluted at 1:1000.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41570

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ATG4C antibody detects ATG4C protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract10 % SDS-PAGEATG4C antibody (GTX129488) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG4C antibody (GTX129488) diluted at 1:1000.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of ATG4C protein from 293T whole cell extracts using 5 £gg of ATG4C antibody (GTX129488).Western blot analysis was performed using ATG4C antibody (GTX129488).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.