Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF6720 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Calcium Activated Nucleotidase 1/CANT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Calcium Activated Nucleotidease 1/CANT1 in direct ELISAs.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q8WVQ1- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Calcium Activated Nucleotidase 1/CANT1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Calcium Activated Nucleotidase 1/CANT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Calcium Activated Nucleotidase 1/CANT1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Calcium Activated Nucleotidase 1/CANT1 in PC-3 Human Cell Line. Calcium Activated Nucleotidase 1/CANT1 was detected in immersion fixed PC-3 human prostate cancer cell line using Sheep Anti-Human Calcium Activated Nucleotidase 1/ CANT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6720) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL011) and counterstained with DAPI (blue). Specific staining was localized to perinuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
