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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB3924 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MafG Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MafG in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafF or rhMafK is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O15525- Isotype
- IgG
- Antibody clone number
- 370412
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MafG by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MafG by Western Blot. Western blot shows recombinant human MafF, MafG, and MafK (5 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
