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LearnPA5-19899
antibody from Invitrogen Antibodies
Targeting: CFLAR
c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
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- Product number
- PA5-19899 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FLIP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~25kDa and ~35kDa. A suggested positive control is Jurkat cell lysate.
- Concentration
- 1 mg/mL
Submitted references TNF-α induces vascular endothelial cells apoptosis through overexpressing pregnancy induced noncoding RNA in Kawasaki disease model.
Jiang C, Fang X, Jiang Y, Shen F, Hu Z, Li X, Huang X
The international journal of biochemistry & cell biology 2016 Mar;72:118-124
The international journal of biochemistry & cell biology 2016 Mar;72:118-124
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cell lysates using a FLIP polyclonal antibody (Product # PA5-19899) at 1:1000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP Polyclonal Antibody (Product # PA5-19899) at 1:1,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of FLIP in Jurkat cells with FLIP Polyclonal Antibody (Product # PA5-19899) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells using FLIP Polyclonal Antibody (Product # PA5-19899) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of FLIP in HeLa cells with FLIP Polyclonal Antibody (Product # PA5-19899) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of FLIP in Jurkat cells with FLIP Polyclonal Antibody (Product # PA5-19899) at 20 µg/mL. Red: FLIP Blue: DAPI staining
