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LearnAF821
antibody from R&D Systems
Targeting: CFLAR
c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF821 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse FLIP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse FLIP in Western blots.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells.
Infrared radiation confers resistance to UV-induced apoptosis via reduction of DNA damage and upregulation of antiapoptotic proteins.
Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis.
Low concentration of interleukin-1beta induces FLICE-inhibitory protein-mediated beta-cell proliferation in human pancreatic islets.
Expression of Fas but not Fas ligand on fetal pig beta cells.
The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
English WR, Ireland-Zecchini H, Baker AH, Littlewood TD, Bennett MR, Murphy G
PloS one 2018;13(4):e0195116
PloS one 2018;13(4):e0195116
Infrared radiation confers resistance to UV-induced apoptosis via reduction of DNA damage and upregulation of antiapoptotic proteins.
Jantschitsch C, Majewski S, Maeda A, Schwarz T, Schwarz A
The Journal of investigative dermatology 2009 May;129(5):1271-9
The Journal of investigative dermatology 2009 May;129(5):1271-9
Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis.
Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J
Blood 2008 Jul 15;112(2):340-9
Blood 2008 Jul 15;112(2):340-9
Low concentration of interleukin-1beta induces FLICE-inhibitory protein-mediated beta-cell proliferation in human pancreatic islets.
Maedler K, Schumann DM, Sauter N, Ellingsgaard H, Bosco D, Baertschiger R, Iwakura Y, Oberholzer J, Wollheim CB, Gauthier BR, Donath MY
Diabetes 2006 Oct;55(10):2713-22
Diabetes 2006 Oct;55(10):2713-22
Expression of Fas but not Fas ligand on fetal pig beta cells.
Bai L, Maedler K, Donath M, Tuch BE
Xenotransplantation 2004 Sep;11(5):426-35
Xenotransplantation 2004 Sep;11(5):426-35
The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
Sade H, Krishna S, Sarin A
The Journal of biological chemistry 2004 Jan 23;279(4):2937-44
The Journal of biological chemistry 2004 Jan 23;279(4):2937-44
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse FLIP by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse FLIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF821) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FLIP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
