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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB7476 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MINA Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MINA in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MINA is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8IUF8- Isotype
- IgG
- Antibody clone number
- 753002
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MINA by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JAR human choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MINA Monoclonal Antibody (Catalog # MAB7476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MINA at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MINA in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MINA Monoclonal Antibody (Catalog # MAB7476, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
