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LearnPAB16930
antibody from Abnova Corporation
Targeting: CCL20
CKb4, exodus-1, LARC, MIP-3a, SCYA20, ST38
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PAB16930 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16930, RRID:AB_10679073
- Product name
- Ccl20 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant Ccl20.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis.
Toll-like receptor 5- and lymphotoxin beta receptor-dependent epithelial Ccl20 expression involves the same NF-kappaB binding site but distinct NF-kappaB pathways and dynamics.
C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE.
Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A
The Journal of investigative dermatology 2009 Sep;129(9):2175-83
The Journal of investigative dermatology 2009 Sep;129(9):2175-83
Toll-like receptor 5- and lymphotoxin beta receptor-dependent epithelial Ccl20 expression involves the same NF-kappaB binding site but distinct NF-kappaB pathways and dynamics.
Sirard JC, Didierlaurent A, Cayet D, Sierro F, Rumbo M
Biochimica et biophysica acta 2009 May;1789(5):386-94
Biochimica et biophysica acta 2009 May;1789(5):386-94
C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE.
Reboldi A, Coisne C, Baumjohann D, Benvenuto F, Bottinelli D, Lira S, Uccelli A, Lanzavecchia A, Engelhardt B, Sallusto F
Nature immunology 2009 May;10(5):514-23
Nature immunology 2009 May;10(5):514-23
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Ccl20 polyclonal antibody (Cat # PAB16930) shows detection of a band ~11 KDa in size corresponding to recombinant mouse Ccl20. Recombinant mouse Ccl20 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
