Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-77532 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- proNGF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references Dexmedetomidine Alleviates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting p75NTR-Mediated Oxidative Stress and Apoptosis.
Wang Z, Wu J, Hu Z, Luo C, Wang P, Zhang Y, Li H
Oxidative medicine and cellular longevity 2020;2020:5454210
Oxidative medicine and cellular longevity 2020;2020:5454210
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof proNGF (WT-mouse, Lanes 1-3) and proNGF (WT-human, Lanes 4-6) with proNGF polyclonal antibody (Product # PA5-77532) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of proNGF (WT-mouse, Lanes 1-3) and proNGF (WT-human, Lanes 4-6) with proNGF polyclonal antibody (Product # PA5-77532) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of proNGF (WT-mouse, Lanes 1-3) and proNGF (WT-human, Lanes 4-6) with proNGF polyclonal antibody (Product # PA5-77532) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of proNGF in rat brain. A) Samples were probed with proNGF polyclonal antibody (Product # PA5-77532) and incubated with GFAP and proNGF. B) Co-localization stained image. C) proNGF stained image.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Dexmedetomidine suppressed apoptosis in AKI induced by LPS. (a, b) TUNEL staining in kidney sections of mice (scale bar = 50 mu m), white arrows to show TUNEL-positive nuclei and quantitation of TUNEL-positive cells. (c, d) Cleaved caspase-3 staining in kidney sections of mice (scale bar = 50 mu m), and quantitation of Cleaved caspase-3-positive cells. (e, f) Western blot analysis and quantitative data for Cleaved caspase-3, p75NTR, proNGF, and NGF in kidney tissues of mice; GAPDH was used as a loading control. (g) p75NTR, NGF, TrkA, and Sortilin gene expression in the kidney tissues of mice ( * p < 0.05 and ** p < 0.01 versus control; # p < 0.05 and ## p < 0.01 versus LPS. Data were presented as mean +- SEM, n = 6).