NB100-65269

antibody from Novus Biologicals

Targeting: CD101 IGSF2, V7

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-65269

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-65269, RRID:AB_959499

Product name

Mouse Monoclonal IGSF2/CD101 Antibody

Antibody type

Monoclonal

Description

Protein G purified. NB100-65269 recognizes human CD101. CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells. Clone BB27 has been reported to inhibit allogeneic T-cell responses (1). Removal of sodium azide is recommended prior to use in functional assays.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD3 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock.