NB100-64770

antibody from Novus Biologicals

Targeting: IL6R CD126

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-64770

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-64770, RRID:AB_959631

Product name

Mouse Monoclonal IL6R Antibody

Antibody type

Monoclonal

Description

Protein A or G purified. Recognizes soluble and membranous human IL-6R. This has been shown to inhibit IL-6 mediated proliferation of XG-1 cells, and partially blocks binding of IL-6 to its receptor. Removal of Sodium azide is recommended prior to use in functional assays.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: IL6R Antibody (B-R6) [NB100-64770] - A surface stain was performed on human peripheral blood lymphocytes with IL6R (B-R6) antibody NB100-64770 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). A co-stain was performed with NBP2-24867AF488.