PAB27463

antibody from Abnova Corporation

Targeting: NME1-NME2 NM23-LV, NMELV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27463

Provider

Abnova Corporation

Product name

NME1-NME2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NME1-NME2.

Antigen sequence

MVLLSTLGIVFQGEGPPISSCDTGTMANCERTFIA
IKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NME1-NME2 polyclonal antibody (Cat # PAB27463) at 1:100-1:250 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with NME1-NME2 polyclonal antibody (Cat # PAB27463) at 1:100-1:500 dilution.Lane 1 : NBT-IILane 2 : PC12

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with NME1-NME2 polyclonal antibody (Cat # PAB27463) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with NME1-NME2 polyclonal antibody (Cat # PAB27463) shows strong cytoplasmic positivity in exocrine glandular cells at 1:20- 1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)