MAB46941

antibody from Novus Biologicals

Targeting: SEMA4A CORD10, FLJ12287, SEMAB, SemB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB46941

Provider

Novus Biologicals

Product name

Mouse Monoclonal Semaphorin 4A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Semaphorin 4A in direct ELISAs and Western blots. In direct ELISA, no cross-reactivity with recombinant human Semaphorin 4B, 4C, or 4D is observed and approximately 5% cross-reactivity with recombinant mouse Semaphorin 4A is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Semaphorin 4A by Western Blot. Western blot shows lysates of human dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Semaphorin 4A Monoclonal Antibody (Catalog # MAB46941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Semaphorin 4A in Mouse Embryo. Semaphorin 4A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Mouse Anti-Human Semaphorin 4A Monoclonal Antibody (Catalog # MAB46941) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.