LS-C353523

antibody from LSBio

Targeting: SEMA4A CORD10, FLJ12287, SEMAB, SemB

Western blot

Immunocytochemistry

Antibody data

Submit

Validation data

SEMA4A protein structure - LS-C353523 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate using 5ug of Anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-Semaphorin 4A Antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate using 5ug of Anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-Semaphorin 4A Antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate using 5ug of Anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-Semaphorin 4A Antibody.