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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- H00064218-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00064218-D01, RRID:AB_10838642
- Product name
- SEMA4A MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human SEMA4A protein.
- Antigen sequence
MALPALGLDPWSLLGLFLFQLLQLLLPTTTAGGGG
QGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLS
GDGNTLYVGAREAILALDIQDPGVPRLKNMIPWPA
SDRKKSECAFKKKSNETQCFNFIRVLVSYNVTHLY
TCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQ
SPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMR
TLGSQPVLKTDNFLRWLHHDASFVAAIPSTQVVYF
FFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQ
KKWTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSP
TAPHIYAVFTSQWQVGGTRSSAVCAFSLLDIERVF
KGKYKELNKETSRWTTYRGPETNPRPGSCSVGPSS
DKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAV
ETAQGLDGHSHLVMYLGTTTGSLHKAVVSGDSSAH
LVEEIQLFPDPEPVRNLQLAPTQGAVFVGFSGGVW
RVPRANCSVYESCVDCVLARDPHCAWDPESRTCCL
LSAPNLNSWKQDMERGNPEWACASGPMSRSLRPQS
RPQIIKEVLAVPNSILELPCPHLSALASYYWSHGP
AAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENG
FSYPVISYWVDSQDQTLALDPELAGIPREHVKVPL
TRVSGGAALAAQQSYWPHFVTVTVLFALVLSGALI
ILVASPLRALRARGKVQGCETLRPGEKAPLSREQH
LQSPKECRTSASDVDADNNCLGTEVA- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SEMA4A expression in transfected 293T cell line (H00064218-T02) by SEMA4A MaxPab polyclonal antibody.Lane 1: SEMA4A transfected lysate(83.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SEMA4A transfected lysate using anti-SEMA4A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SEMA4A purified MaxPab mouse polyclonal antibody (B01P) (H00064218-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
