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Immunoprecipitation
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 302014 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#302014, RRID:AB_314214
- Product name
- LEAF™ Purified anti-human CD16 [3G8]
- Antibody type
- Monoclonal
- Antigen
- CD16
- Description
- CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. It is over 95% homologous to CD16a in extracellular domain and is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 1 mg/ml
- Storage
- The CD16 antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Submitted references Bi-specific aptamers mediating tumor cell lysis.
Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice.
Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?
IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa.
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B
The Journal of biological chemistry 2011 Jun 17;286(24):21896-905
The Journal of biological chemistry 2011 Jun 17;286(24):21896-905
Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice.
Brainard DM, Seung E, Frahm N, Cariappa A, Bailey CC, Hart WK, Shin HS, Brooks SF, Knight HL, Eichbaum Q, Yang YG, Sykes M, Walker BD, Freeman GJ, Pillai S, Westmoreland SV, Brander C, Luster AD, Tager AM
Journal of virology 2009 Jul;83(14):7305-21
Journal of virology 2009 Jul;83(14):7305-21
Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?
Timmerman KL, Flynn MG, Coen PM, Markofski MM, Pence BD
Journal of leukocyte biology 2008 Nov;84(5):1271-8
Journal of leukocyte biology 2008 Nov;84(5):1271-8
IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa.
Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK
Blood 2008 May 15;111(10):5037-46
Blood 2008 May 15;111(10):5037-46
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained with LEAF™ purified 3G8, followed by anti-mouse IgGs FITC
- Conjugate
- Unconjugated
