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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- AF1597 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fc gamma RIIIA/B (CD16) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human Fc gamma RIIA is observed and less than 2% cross-reactivity with recombinant mouse CD16 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O75015- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Microvesicles released by apoptotic human neutrophils suppress proliferation and IL-2/IL-2 receptor expression of resting T helper cells.
Multivalent Fcγ-receptor engagement by a hexameric Fc-fusion protein triggers Fcγ-receptor internalisation and modulation of Fcγ-receptor functions.
Induced expression of FcγRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-γhigh subset.
Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells.
α-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.
Shen G, Krienke S, Schiller P, Nießen A, Neu S, Eckstein V, Schiller M, Lorenz HM, Tykocinski LO
European journal of immunology 2017 May;47(5):900-910
European journal of immunology 2017 May;47(5):900-910
Multivalent Fcγ-receptor engagement by a hexameric Fc-fusion protein triggers Fcγ-receptor internalisation and modulation of Fcγ-receptor functions.
Qureshi OS, Rowley TF, Junker F, Peters SJ, Crilly S, Compson J, Eddleston A, Björkelund H, Greenslade K, Parkinson M, Davies NL, Griffin R, Pither TL, Cain K, Christodoulou L, Staelens L, Ward E, Tibbitts J, Kiessling A, Smith B, Brennan FR, Malmqvist M, Fallah-Arani F, Humphreys DP
Scientific reports 2017 Dec 6;7(1):17049
Scientific reports 2017 Dec 6;7(1):17049
Induced expression of FcγRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-γhigh subset.
Chauhan AK, Chen C, Moore TL, DiPaolo RJ
The Journal of biological chemistry 2015 Feb 20;290(8):5127-5140
The Journal of biological chemistry 2015 Feb 20;290(8):5127-5140
Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells.
Chauhan AK, Moore TL
Clinical and experimental immunology 2012 Feb;167(2):235-45
Clinical and experimental immunology 2012 Feb;167(2):235-45
α-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.
Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG
The Journal of clinical investigation 2010 Dec;120(12):4236-50
The Journal of clinical investigation 2010 Dec;120(12):4236-50
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Fc gamma RIII (CD16) by Western Blot. Western blot shows lysates of human spleen tissue, human lung tissue, and human peripheral blood mononuclear cells (PBMC) . PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Fc gamma RIII (CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1597) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fc gamma RIII (CD16) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
