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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-14789 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HK1 Monoclonal Antibody (R.457.3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- R.457.3
- Vial size
- 100 µL
- Concentration
- 127 µg/mL
- Storage
- -20°C
Submitted references Defective Mitochondrial Pyruvate Flux Affects Cell Bioenergetics in Alzheimer's Disease-Related Models.
Effect of lentivirus-mediated shRNA inactivation of HK1, HK2, and HK3 genes in colorectal cancer and melanoma cells.
Rossi A, Rigotto G, Valente G, Giorgio V, Basso E, Filadi R, Pizzo P
Cell reports 2020 Feb 18;30(7):2332-2348.e10
Cell reports 2020 Feb 18;30(7):2332-2348.e10
Effect of lentivirus-mediated shRNA inactivation of HK1, HK2, and HK3 genes in colorectal cancer and melanoma cells.
Kudryavtseva AV, Fedorova MS, Zhavoronkov A, Moskalev AA, Zasedatelev AS, Dmitriev AA, Sadritdinova AF, Karpova IY, Nyushko KM, Kalinin DV, Volchenko NN, Melnikova NV, Klimina KM, Sidorov DV, Popov AY, Nasedkina TV, Kaprin AD, Alekseev BY, Krasnov GS, Snezhkina AV
BMC genetics 2016 Dec 22;17(Suppl 3):156
BMC genetics 2016 Dec 22;17(Suppl 3):156
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), A-431 (Lane 2), U-87 MG (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Panc-1 (Lane 6) and Caki-1 (Lane 7). The blot was probed with Anti-HK1 Monoclonal Antibody (Product # MA5-14789, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 102 kDa band corresponding to HK1 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), A-431 (Lane 2), U-87 MG (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Panc-1 (Lane 6) and Caki-1 (Lane 7). The blot was probed with Anti-HK1 Monoclonal Antibody (Product # MA5-14789, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 102 kDa band corresponding to HK1 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hexokinase I in HeLa cells using a Hexokinase I monoclonal antibody (Product # MA5-14789) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Hexokinase I in paraffin-embedded human lung carcinoma using a Hexokinase I monoclonal antibody (Product # MA5-14789).
