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antibody from Invitrogen Antibodies
Targeting: RNF2
BAP-1, BAP1, DING, HIPI3, RING1B, RING2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-96361 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MSQAVQTNGT QPLSKTWELS LYELQRTPQE AITDGLEIVV SPRSLHSELM CPICLDMLKN TMTTKECLHR FCADCIITAL RSGNKECPTC RKKLVSKRSL RPDPNFDALI SKIYPSRDEY EAHQERVLAR INKHNNQQAL SHSIEEGLKI QAMNRLQRGK KQQIENGSGA EDNGDSSHCS NASTHSNQEA GPSNKRTKTS DDSGLELDNN NAAMAIDPVM DGASEIELVF RPHPTLMEKD DSAQTRYIKT SGNATVDHLS KYLAVRLALE ELRSKGESNQ MNLDTASEKQ YTIYIATASG QFTVLNGSFS LELVSEKYWK VNKPMELYYA PTKEHK; Positive Samples: 293T, Jurkat, U-937, SH-SY5Y, Mouse brain, Mouse spleen; Cellular Location: Chromosome, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RNF2 in extracts of various cell lines using RNF2 Polyclonal Antibody (Product # PA5-96361) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RNF2 in paraffin-embedded rat lung using RNF2 Polyclonal Antibody (Product # PA5-96361) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RNF2 in paraffin-embedded human gastric cancer using RNF2 Polyclonal Antibody (Product # PA5-96361) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RNF2 in paraffin-embedded mouse brain using RNF2 Polyclonal Antibody (Product # PA5-96361) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of RNF2 in HeLa using RNF2 Polyclonal Antibody (Product # PA5-96361) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RNF2 was performed in 200 µg extracts of 293T cells using RNF2 Polyclonal Antibody (Product # PA5-96361). Western blot was performed from the immunoprecipitate using RNF2 Polyclonal Antibody at a dilution of 1:1000.
