AB-12-NA

antibody from Novus Biologicals

Targeting: TGFB2

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-12-NA

Provider

Novus Biologicals

Product name

Rabbit Polyclonal TGF-beta 2 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects TGF-beta 2 and TGF-beta 1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with TGF-beta 3 and TGF-beta 1 is observed.

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

MicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2.

Howe GA, Kazda K, Addison CL
PloS one 2017;12(10):e0185619

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Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFβ2/TβR and CD44 in MDA-MB-231 breast cancer cells.

Reithmeier A, Panizza E, Krumpel M, Orre LM, Branca RMM, Lehtiö J, Ek-Rylander B, Andersson G
BMC cancer 2017 Sep 15;17(1):650

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Cancer-associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-β-dependent pathways in genotype-specific oral cancer.

Cirillo N, Hassona Y, Celentano A, Lim KP, Manchella S, Parkinson EK, Prime SS
Carcinogenesis 2017 Jan;38(1):76-85

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Differential versican isoforms and aggrecan expression in the chicken embryo aorta.

Arciniegas E, Neves CY, Candelle D, Parada D
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 2004 Jul;279(1):592-600

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Isoform specificity of commercially-available anti-TGF-beta antibodies.

Mozes MM, Hodics T, Kopp JB
Journal of immunological methods 1999 May 27;225(1-2):87-93

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Isoform specificity of commercially-available anti-TGF-beta antibodies.

Mozes MM, Hodics T, Kopp JB
Journal of immunological methods 1999 May 27;225(1-2):87-93

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TGF-beta 2 by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 2 at approximately 64 kDa (as indicated) using 5 µg/mL of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TGF-beta 2 by Western Blot. Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TGF-beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

TGF-beta 2 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 2 Antibody. Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA). The ND₅₀ is typically 0.25-1.25 µg/mL.