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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
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- Product number
- 703010 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 53BP2 Recombinant Rabbit Monoclonal Antibody (8H3L19)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Pig, Bovine, Sheep.
- Antibody clone number
- 8H3L19
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, Caco-2 cells were fixed and permeabilized for detection of endogenous 53BP2 using Anti-53BP2 Recombinant Rabbit Monoclonal Antibody (Product # 703010, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of 53BP2 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating junctional localization of 53BP2. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of 53BP2 was achieved by transfecting Caco-2 cells with 53BP2 specific siRNA (Silencer® select Product # s14316 & s14317). Immunofluorescence analysis was performed on Caco-2 cells (untransfected, panel a-d), transfected with 53BP2 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-53BP2 Recombinant Rabbit Monoclonal Antibody (Product # 703010, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of signal was observed upon siRNA mediated knockdown (panel i-l) confirming specificity of the antibody to 53BP2 (green). The images were captured at 60X magnification.
