Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B335 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ CAMK4 / CaMK IV Antibody LS-B335
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Rabbit
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations. Staining of rat brain lysate is shown in lane 1. Jurkat cell lysate staining is shown in lane 2.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 1 and 2 respectively).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-CaM Kinase IV Antibody - Western Blot. Western blot of Anti-CaM Kinase IV antibody shows detection of a band ~52 kD corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1000 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.