Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-48255 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mimecan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with bovine based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The proteoglycan mimecan is associated with carotid plaque vulnerability and increased risk of future cardiovascular death.
Tengryd C, Nielsen SH, Cavalera M, Bengtsson E, Genovese F, Karsdal M, Dunér P, Orho-Melander M, Nilsson J, Edsfeldt A, Gonçalves I
Atherosclerosis 2020 Nov;313:88-95
Atherosclerosis 2020 Nov;313:88-95
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OGN in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OGN gene. Lysates were probed with a OGN polyclonal antibody (Product # PA5-48255).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OGN in in CEM cell line lysates (35µg/lane). Samples were probed with a OGN Antibody (C-term) (Product # PA5-48255).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of OGN in formalin fixed and paraffin embedded human uterus tissue. Samples stained using a OGN Antibody (C-term) (Product # PA5-48255), followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of OGN in 293 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a OGN Antibody (C-term) (Product # PA5-48255). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
