Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX83704 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83704, RRID:AB_10729399
- Product name
- RNF144B antibody [9H10]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Anti-RNF144B mouse monoclonal antibody (GTX83704) immunofluorescent staining of COS7 cells transiently transfected with RNF144B
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (GTX83704).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-RNF144Bmouse monoclonal antibody. (GTX83704, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-RNF144Bmouse monoclonal antibody. (GTX83704, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-RNF144Bmouse monoclonal antibody. (GTX83704, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-RNF144Bmouse monoclonal antibody. (GTX83704, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue using anti-RNF144Bmouse monoclonal antibody. (GTX83704, Dilution 1:50)
Supportive validation
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- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-RNF144B antibody(GTX83704),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY RNF144B