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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA500186 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500186, RRID:AB_2108898
- Product name
- GATA6 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3)
- Antibody type
- Monoclonal
- Description
- GATA6 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GATA6
- Isotype
- IgG
- Antibody clone number
- OTI2B3
- Vial size
- 100 µl
- Concentration
- 0.3 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA6 (RC220721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA6.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-GATA6 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-GATA6 mouse monoclonal antibody (TA500186).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GATA6 mouse monoclonal antibody (TA500186) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA6(RC220721).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-GATA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500186)
- Validation comment
- IHC
