M13632

antibody from Boster Biological Technology

Targeting: HSPA12A FLJ13874, KIAA0417

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M13632

Provider

Boster Biological Technology

Product name

Anti-HSPA12A Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for HSPA12A detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat HSPA12A information: Molecular Weight: 74978 MW; Tissue Specificity: Widely expressed with highest levels in brain, kidney and muscle.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

BDD-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse brain, using HSPA12A Antibody(M13632)HSPA12A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA12A Antibody (M13632)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image