Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-47692 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ninjurin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 10% cross-reactivity with recombinant human Ninjurin-1 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis from lysates of NTera-2 human testicular embryonic carcinoma cell line, mouse spleen tissue and rat thymus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human Ninjurin-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47692) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Ninjurin-2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ninjurin 2 in NTera‚2 human testicular embryonic carcinoma cell line, mouse spleen tissue and rat thymus tissue. Samples were incubated in Ninjurin 2 polyclonal antibody (Product # PA5-47692) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Ninjurin‚2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions.