H00005179-M04

antibody from Abnova Corporation

Targeting: PENK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005179-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005179-M04, RRID:AB_10757356

Product name

PENK monoclonal antibody (M04), clone 9E7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant PENK.

Antigen sequence

MARFLTLCTWLLLLGPGLLATVRAECSQDCATCSY
RLVRPADISFLACVMECEGKLPSLKIWETCKELLQ
LSRPELPQDGTSTLRENSKPEESHLLAKRYGGFMK
RYGGFMKKMDELYPMEPEEEANGSEILAKRYGGFM
KKDAEEDDSLANSSDLLKELLETGDNRERSHHQDG
SDNEEEVSKRYGGFMRGLKRSPQLEDEAKELQKRY
GGFMRRVGRPEWWMDYQKRYGGFLKRFAEALPSDE
EGESYSKEVPEMEKRYGGFMRF


Isotype

IgG

Antibody clone number

9E7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PENK expression in transfected 293T cell line by PENK monoclonal antibody (M04), clone 9E7.Lane 1: PENK transfected lysate (Predicted MW: 29.48 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PENK is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol