AP5592a

antibody from Abgent

Targeting: ZDHHC2 DHHC2, ZNF372

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP5592a

Provider

Abgent

Proper citation

Abgent Cat#AP5592a, RRID:AB_10838868

Product name

ZDHC2 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ZDHC2 Antibody (N-term) (Cat. #AP5592a) western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).This demonstrates the ZDHC2 antibody detected the ZDHC2 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of ZDHC2 Antibody (N-term) Pab (Cat. #AP5592a) pre-incubated without(lane 1) and with(lane 2) blocking peptide in Ramos cell line lysate. ZDHC2 Antibody (N-term) (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ZDHC2 Antibody (N-term) (Cat. #AP5592a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZDHC2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ZDHC2 Antibody (N-term) (Cat. #AP5592a) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50