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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- orb137624 - Provider product page
- Provider
- Biorbyt
- Product name
- TPPP antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal to TPPP.
- Reactivity
- Human, Simian
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 μg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20°C. Minimize freezing and thawing.
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Supportive validation
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- Biorbyt (provider)
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- Experimental details
- Immunohistochemical staining of paraffin embedded human brain tissue using TPPP antibody (primary antibody at 1:200)
- Other comments
- Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 26kD band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kD according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01 μg/ml. An additional band of unknown identity was also consistently observed at 17kD. This band was successfully blocked by incubation with the immunizing peptide.
