Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 13850-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCAM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: VELSVGESK FFTCTAIGEP ESIDWYNPQG EKIISTQRVV VQKEGVRSRL TIYNANIEDA GIYRCQATDA KGQTQEATVV LEIYQKLTFR EVVSPQEFKQ GEDAEVVCRV SSSPAPAVSW LYHNEEVTTI SDNRFAMLAN NNLQILNINK SDEGIYRCEG RVEARGEIDF RDIIVIVNVP PAISMPQKSF NATAERGEEM TFSCRASGSP EPAISWFRNG KLIEENEKYI LKGSNTELTV RNIINSDGGP YVCRATNKAG EDEKQAFLQV FVQPHIIQLK NETTYENGQV TLVCDAEGEP IPEITWKRAV DGFTFTEGDK SLD (30-351 aa encoded by BC052946)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 13850-1-AP (NCAM2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 13850-1-AP (NCAM2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13850-1-AP ( NCAM2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13850-1-AP ( NCAM2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NCAM2 (IP:13850-1-AP, 3ug; Detection:13850-1-AP 1:1000) with mouse brain tissue lysate 4000ug.