HPA014975

antibody from Atlas Antibodies

Targeting: ST6GALNAC1 SIAT7A, ST6GalNAcI

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA014975

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA014975, RRID:AB_1857529

Product name

Anti-ST6GALNAC1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PQTKPSRHQRTENIKERSLQSLAKPKSQAPTRARR
TTIYAEPVPENNALNTQTQPKAHTTGDRGKEANQA
PPEEQDKVPHTAQRAAWKSPEKEKTMVNTLSPRGQ
DAGMASGRTEAQSWKSQDTKTTQGN


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human rectum and skeletal muscle tissues using HPA014975 antibody. Corresponding ST6GALNAC1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows strong positivity in Golgi apparatus in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows strong positivity in Golgi apparatus in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong positivity in Golgi apparatus in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.

Sample type

HUMAN