Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-267-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human NT-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human NT-3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) NT-4 and rhBDNF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P20783
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Saygili E, Schauerte P, Küppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schröder JW, Marx N, Rana OR
Journal of molecular and cellular cardiology 2010 Jul;49(1):79-87
Journal of molecular and cellular cardiology 2010 Jul;49(1):79-87
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NT-3 in Rat Brain. NT-3 was detected in perfusion fixed frozen sections of rat brain (Purkinje cells in the cerebellum) using 10 µg/mL Human NT-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-267-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by NT-3 and Neutralization by Human NT-3 Antibody. Recombinant Human NT-3 (Catalog # 267-N3) stimulates proliferation in BaF-TrKB-BD mouse pro-B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT-3 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human NT-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-267-NA). The ND50 is typically 0.1-0.4 µg/mL.