PA5-28086

antibody from Invitrogen Antibodies

Targeting: PSMB5 MB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28086

Provider

Invitrogen Antibodies

Product name

PSMB5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, H1299.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSMB5 using 30 µg of A) A431 and B) H1299 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a PSMB5 polyclonal antibody (Product # PA5-28086) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PSMB5 was performed by separating 30 µg of various whole cell extracts by 12% SDS PAGE. Proteins were transferred to a membrane and probed with a PSMB5 Polyclonal Antibody (Product # PA5-28086) at a dilution of 1:1000. A: A431, B: H1299.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PSMB5 Rabbit Polyclonal Antibody (Product # PA5-28086) and 21 kDa band corresponding to PSMB5 was observed across cell lines tested and was also decreased upon IFN gamma treatment in HeLa cells. Whole cell extracts (30 µg lysate) of HeLa (Lane1), HeLa treated with IFN gamma (100 ng/mL for 72h) (Lane2), DU 145 (Lane 3), DU 145 treated with resveratrol (75uM for 24h) (Lane 4), MCF-7 (Lane 5), Hep G2 (Lane 6), A-431 (Lane 7) and SK-O-V3 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0332BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PSMB5 in paraformaldehyde-fixed A431 cells using a PSMB5 polyclonal antibody (Product # PA5-28086) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PSMB5 (Product # PA5-28086) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.