361001

antibody from BioLegend

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

361001

Provider

BioLegend

Proper citation

BioLegend Cat#361001, RRID:AB_2562882

Product name

Purified anti-human CD146

Antibody type

Monoclonal

Antigen

Cultured human umbilical cells

Description

CD146 is a 118 kD integral transmembrane glycoprotein also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

P1H12

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human cervical cancer cell line HeLa was stained with purified anti-human CD146 (clone P1H12, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.

Conjugate

Unconjugated