H00010361-M03

antibody from Abnova Corporation

Targeting: NPM2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010361-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010361-M03, RRID:AB_10963130

Product name

NPM2 monoclonal antibody (M03), clone 5E9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant NPM2.

Antigen sequence

MNLSSASSTEEKAVTTVLWGCELSQERRTWTFRPQ
LEGKQSCRLLLHTICLGEKAKEEMHRVEILPPANQ
EDKKMQPVTIASLQASVLPMVSMVGVQLSPPVTFQ
LRAGSGPVFLSGQERYEASDLTWEEEEEEEGEEEE
EEEEDDEDEDADISLEEQSPVKQVKRLVPQKQASV
AKKKKLEKEEEEIRASVRDKSPVKKAKATARAKKP
GFKK


Isotype

IgG

Antibody clone number

5E9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NPM2 expression in transfected 293T cell line by NPM2 monoclonal antibody (M03), clone 5E9.Lane 1: NPM2 transfected lysate (Predicted MW: 24.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NPM2 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to NPM2 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol