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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- OAAB17245 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#OAAB17245, RRID:AB_2630500
- Product name
- HRAS antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of anti-HRAS Antibody (Center) (Cat.# OAAB17245) in Jurkat cell line lysates (35µg/lane). HRAS (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of HRAS Antibody (Center) (Cat# OAAB17245) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) .Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red) .DAPI was used to stain the cell nuclear (blue) .
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
