AM32521PU-N

antibody from Acris Antibodies GmbH

Targeting: HRAS HRAS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM32521PU-N

Provider

Acris Antibodies GmbH

Product name

anti HRAS (+NRAS)

Antibody type

Monoclonal

Antigen

Full length recombinant GST-tagged Human H-Ras.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1A6.2

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Ras Isoform Western Blot Analysis:Representative lot data. Recombinant N-Ras, K-Ras, and H-Ras proteins and A431 lysate (Lanes 1, 2, 3, and 4, respectively) were resolved by SDS-PAGE, transferred to PVDF, and probed with anti-Ras, clone 1A6. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicates Ras.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Species Cross-Reactivity Western Blot Analysis:Mouse brain (lane 1), Rat Brain (lane 2), and A431 (lane 3) cell lysates were resolved by SDS-PAGE, transferred to PVDF, and probed with anti-Ras, clone 1A6. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Ras.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot Analysis:Rat brain lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with anti-Ras, clone 1A6.2 (1/1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Ras. 

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry:Top: Representative lot data. Representative staining pattern/morphology on stratified Squamous epithelium in human tonsil. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. Antibody was diluted to 1:50, IHC Select Detection System HRP-DAB.Bottom:Representative lot data. Negative staining control representative staining pattern/morphology on stratified Squamous epithelium in human tonsil. Tissue pretreated with Citrate pH 6.0, antigen retrieval. Antibody was omitted, IHC-Select Detection with HRPDAB. Immunoreactivity was not detectable.