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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA505666 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505666, RRID:AB_2623267
- Product name
- HRAS mouse monoclonal antibody,clone OTI1C8
- Antibody type
- Monoclonal
- Description
- HRAS mouse monoclonal antibody,clone OTI1C8
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HRAS
- Isotype
- IgG
- Antibody clone number
- OTI1C8
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-HRAS monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-HRAS monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HRAS mouse monoclonal antibody (TA505666) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505666)
- Validation comment
- IHC
