H00003265-B01P

antibody from Abnova Corporation

Targeting: HRAS HRAS1

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003265-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003265-B01P, RRID:AB_1674025

Product name

HRAS purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human HRAS protein.

Antigen sequence

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPT
IEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQ
YMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIP
YIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPD
ESGPGCMSCKCVLS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HRAS expression in transfected 293T cell line (H00003265-T02) by HRAS MaxPab polyclonal antibody.Lane 1: HRAS transfected lysate(20.79 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PIK3R1 and HRAS. HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-HRAS mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)