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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- PA5-52741 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DCT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NECDVCTDQL FGAARPDDPT LISRNSRFSS WETVCDSLDD YNHLVTLCNG TYEGLLRRNQ MGRNSMKLPT LKDIRDCLSL QKFDNPPFFQ NSTFSFRNAL EGFDKADGTL DSQVMSLHNL VHSFLNGTNA LPHSAANDPI FVVLHSFTDA Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 52%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCT in control (vector only transfected HEK293T lysate) and DCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DCT Polyclonal Antibody (Product # PA5-52741).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DCT in human cell line A-431 shows positivity in nucleus. Samples were probed using a DCT Polyclonal Antibody (Product # PA5-52741).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DCT in human lymph node using DCT Polyclonal Antibody (Product # PA5-52741).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DCT in human cerebral cortex using DCT Polyclonal Antibody (Product # PA5-52741).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DCT in human skin shows strong cytoplasmic positivity in melanocytes. Samples were probed using a DCT Polyclonal Antibody (Product # PA5-52741).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DCT in human testis using DCT Polyclonal Antibody (Product # PA5-52741).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DCT in human skin and skeletal muscle tissues using DCT Polyclonal Antibody (Product # PA5-52741). Corresponding DCT RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DCT in human cerebral cortex, lymph node, skin and testis using DCT Polyclonal Antibody (Product # PA5-52741) (A) shows similar protein distribution across tissues to an independent DCT Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DCT in human skeletal muscle using DCT Polyclonal Antibody (Product # PA5-52741) shows low expression as expected.
