PAB24144

antibody from Abnova Corporation

Targeting: NOP2 NOL1, NOP120, NSUN1, p120

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB24144

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB24144, RRID:AB_11123003

Product name

NOP2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NOP2.

Antigen sequence

KLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRR
RDLAQALINRGVNLDPLGKWSKTGLVVYDSSVPIG
ATPEYLAGH


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NOP2 polyclonal antibody (Cat # PAB24144) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with NOP2 polyclonal antibody (Cat # PAB24144) at 1-4 ug/mL dilution shows positivity in nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human skin with NOP2 polyclonal antibody (Cat # PAB24144) shows distinct nucleolar positivity in keratinocytes at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)