Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C669611 - Provider product page
- Provider
- LSBio
- Product name
- ACAA2 Antibody LS-C669611
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue. All lanes: ACAA2 antibody at 2.4 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42 KDa. Observed band size: 42 KDa
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue. All lanes: ACAA2 antibody at 2.4 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42 KDa. Observed band size: 42 KDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating ACAA2 in A549 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ACAA2 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ACAA2 Antibody (6µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ACAA2 in A549 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ACAA2 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ACAA2 Antibody (6µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ACAA2 in A549 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ACAA2 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ACAA2 Antibody (6µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (10µg)